Thin Layer Chromatography

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Introduction

Summary

TLC is a quick technique for separating and identifying compounds present in a mixture by having them pass through a fixed, inert medium (alumina, silica…) with the aid of a mobile solvent (gas or liquid) which carries them through the medium. Each component of the mixture travels through the medium at a different rate as a result of its solubility in the solvent and its affinity for the fixed medium.

TLC is also commonly used to assess the effectiveness of a purification.

Goals

To understand how TLC is carried out.
To understand how Rf is determined from the raw results on a TLC plate

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