Gene cloning

Ages

Introduction

Summary

Cloning a gene is multi-step process involving  the isolation of the gene of interest from one given organism, then the transfer of the gene of interest to a host cell which is capable of  producing  large quantities of recombinant protein.

Goals

• To illustrate the different steps required to clone a gene of interest
• To understand how a gene can be transferred to a host cell
• To understand how the host cells having the recombinant DNA are selected

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Tags

Recombinant DNA genetic-engineering plasmid bacteria transformation chromosome cloning promoter antibiotic restriction-enzyme endonuclease gene ligase biotechnology