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Direct enzyme-linked immunosorbent assay (ELISA) HTML5

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Summary

ELISA, Enzyme Linked Immunosorbent Assay, is a is an immuno-enzymatic technique that enables the detection of antigen-antibody reactions thanks to a reaction with colored products. ELISA is a technique currently used in the process of screening for HIV infections.

Learning goals

  • To understand the principle behind the use of the ELISA technique in screening for AIDS.
  • To describe the different steps invloved in the ELISA technique.
  • To point out the differences between direct ELISA and indirect ELISA.

 

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Antibodies (Ab) are secreted by the plasmacytes after a given antigen (Ag), a virus for example, is introduced into the organism. The presence of an antigen, or of its corresponding antibody, in a…

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