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Dilution HTML5


In laboratories, it is common practice to prepare a solution from a concentrated mother solution.

Example: one wants to prepare a volume V = 100 mL of a daughter solution of concentration  C = 0.010 mol.L-1 from a mother solution with a concentration  C = 0.10 mol.L-1.

The preparation technique for such a solution is to prepare a dilution. This simple procedure is carried out using a very precise  experimental protocol.

  • Some reagents are corrosive. They have to be handled using gloves and goggles.
  • Glassware (vials, flasks, pipettes, etc) have to be cleaned in advance.
  • The pipette must always be manipulated in a vertical position. When the contents of the pipette are being transferred to another container, the recipient vessel must be inclined so that it is in contact with the point of the pipette.
  • Readings from graduated glassware  are made with the eye at the same level as the bottom of the meniscus.
  • The used pipette must be placed  on absorbent paper.
  • The recipient vessel must, at the end of the procedure, be labeled. The label must give the name of the solution and its concentration.

Learning goals

  • To  describe the list of materials needed to carry out the preparation of a solution.
  • To  illustrate each of the steps in carrying out a dilution.
  • To know how to carry out the experimental protocol.

Learn more

What goes on during a dilution?

The dilution of a solution is obtained  by adding distilled water of a given volume to the original, or “mother” solution. In other words, the amount of matter…

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