Gene cloning

Summary

Cloning a gene is multi-step process involving  the isolation of the gene of interest from one given organism, then the transfer of the gene of interest to a host cell which is capable of  producing  large quantities of recombinant protein.

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Learning goals

• To illustrate the different steps required to clone a gene of interest
• To understand how a gene can be transferred to a host cell
• To understand how the host cells having the recombinant DNA are selected

Keywords

• antibiotic
• bacteria
• biotechnology
• chromosome
• cloning
• dna
• endonuclease
• gene
• genetic-engineering
• ligase
• plasmid
• promoter
• restriction-enzyme
• transformation

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