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Cloning a gene is multi-step process involving the isolation of the gene of interest from one given organism, then the transfer of the gene of interest to a host cell which is capable of producing large quantities of recombinant protein.
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- To illustrate the different steps required to clone a gene of interest
- To understand how a gene can be transferred to a host cell
- To understand how the host cells having the recombinant DNA are selected
The stages of a gene cloning technology are :
- Cleaving DNA: this stage requires a restriction enzyme that specifically cuts the genomic DNA containing the gene of interest as well as the DNA…
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