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Gene cloning

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Summary

Cloning a gene is multi-step process involving  the isolation of the gene of interest from one given organism, then the transfer of the gene of interest to a host cell which is capable of  producing  large quantities of recombinant protein.

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Learning goals

  • To illustrate the different steps required to clone a gene of interest
  • To understand how a gene can be transferred to a host cell
  • To understand how the host cells having the recombinant DNA are selected

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The stages of a gene cloning technology are :

  1. Cleaving DNA: this stage requires a restriction enzyme that specifically cuts the genomic DNA containing the gene of interest as well as the DNA…

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